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CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response.
来源:Nature medicine. 2018 Jun 11.   作者:Haapaniemi E, Botla S, Persson J, Schmierer B, Taipale   发布时间:2018/6/14 9:12:35

Here, we report that genome editing by CRISPR-Cas9 induces a p53-mediated DNA damage response and cell cycle arrest in immortalized human retinal pigment epithelial cells, leading to a selection against cells with a functional p53 pathway. Inhibition of p53 prevents the damage response and increases the rate of homologous recombination from a donor template. These results suggest that p53 inhibition may improve the efficiency of genome editing of untransformed cells and that p53 function should be monitored when developing cell-based therapies utilizing CRISPR-Cas9.

 

 

CRISPR-Cas9 基因组编辑能够诱导p53介导的DNA损伤应答及细胞周期停滞。

 

 

PMID:
 
29892067
 
DOI:
 
10.1038/s41591-018-0049-z

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